Figure 6. MiR-143 sensitized the PC cells to docetaxel treatment in vivo.

(A) PC-3 cells stably expressing miR-143 or miR-NC at 5x10⁶ cells were subcutaneously injected into both flanks of male BALB/cA nude mice. Tumor volume was monitored over time as indicated, then IGF-I (200 ng/ml) and docetaxel (10 nM) was administrated by peritoneal injection. (B) After 28 days post implantation, the xenografts were harvested and photographed. Representative pictures from each group were shown. Bar=2 mm. (C) The tumors from each group were weighed and the results showed miR-143 overexpression decreased tumor weight in vivo. (D) Total RNAs were isolated and assayed by RT-qPCR to determine the expression of VEGF in tumors. Data represent mean ± SD. ^* indicates significant difference at P<0.05; ^** indicates significant difference at P<0.01.