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. 2017 Nov 18;8(63):107188–107205. doi: 10.18632/oncotarget.22513

Figure 6. The inhibition of c-MET or EGFR leads to BCRP reduction and doxorubicin sensitization.

Figure 6

(A) The scSKOV3 cells were incubated with PI3K inhibitor (LY294; 5 μM) and protein levels for BCRP were assessed by Western blotting. (B-C) After incubation of the scSKOV3 with pharmacological inhibitor of c-MET (SU112; 1 μM) or EGFR (LAPA; 2 μM, GEF; 5 μM), BCRP protein levels were determined. (D-E) The scSKOV3 cells were incubated with SU112 or LAPA, and cellular accumulation levels of Hoechst 33342 (H342; 2 μg/ml for 30 min; D) or doxorubicin (Dox; 2 μM for 6 h; E) were monitored. Cellular fluorescent intensities were quantified using a Cell Insight system. Values are means ± SD from 4 experiments. (F) The scSKOV3 cells were transiently transfected with c-MET-specific siRNA (siMET) or EGFR-specific siRNA (siEGFR), and the protein level for BCRP was assessed by Western blotting. (G) Transcript levels of BCRP were monitored using real-time PCR analysis after transfection with c-MET-specific siRNA (siMET) or EGFR-specific siRNA (siEGFR). Values are means ± SD from three experiments. Similar blots were obtained from three independent experiments (A, B, C and F).