Table 1.
Voltage-dependence of activation, fast and slow inactivation of BgNav1-1a and mutant channels at the holding potential of -120 mV.
| Activation | Fast Inactivation | Slow Inactivation | ||||
|---|---|---|---|---|---|---|
|
| ||||||
| V1/2 (mV) | k | V1/2 (mV) | k | V1/2 (mV) | k | |
| BgNav1-1a | -28.2 ± 0.5 | 5.1 ± 0.9 | -49.0 ± 0.5 | 4.2 ± 0.1 | -54.6 ± 0.9 | 4.2 ± 0.2 |
| F4i15Y | -30.7 ± 1.3 | 5.4 ± 0.4 | -48.4 ± 0.5 | 4.0 ± 0.1 | -58.5 ± 1.3 | 5.5 ± 0.3 |
| V4i18I | -27.4 ± 1.4 | 5.4 ± 0.6 | -46.8 ± 0.5 | 4.1 ± 0.1 | -56.7 ± 1.5 | 3.9 ± 0.2 |
| V4i18A | -33.6 ±1.5 | 6.3 ± 0.9 | -50.0 ± 1.1 | 4.9 ± 0.5 | 60.7 ± 0.4 | 3.7 ± 0.3 |
The voltage dependences of conductance and inactivation were fitted with a two-state Boltzmann equation to determine V1/2, the voltage for half- maximal conductance or inactivation, and k, the slope factor for conductance or inactivation. The values in the table represent the mean ± S.E.M. and the number of oocytes was 6-13.