Figure 2.

ADAM10^DC−/− mice have diminished T_H2 responses to HDM. ADAM10^DC−/− and WT mice were subjected to HDM or saline sensitization and challenge. A. Airway hyperresponsivness was assessed by nebulizing increasing doses of methacholine and measuring resistance. B. Bronchoalveolar lavage fluid (BALF) was harvested and analyzed by flow cytometry. C. and D. Il4 and Il13 mRNA expression was measured in lung tissue relative to Gapdh. E. Formalin fixed lung sections were stained with H&E. F. – H. HDM-specific IgG1, total IgG1, and total IgE were measured in serum by ELISA. Symbols represent individual mice. Data are combined from three independent experiments with n = 10 per HDM group and n = 3 per saline group. Data are presented as mean ± SEM. ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05, ANOVA with Tukey’s post hoc test (A, B), unpaired Student’s t test (C, D, F, G), and Mann-Whitney test (H).