Figure 3.

ADAM10^DC−/− mice are resistant to active systemic anaphylaxis. WT and ADAM10^DC−/− mice were immunized for the ASA protocol and then challenged with OVA on day 25 after immunization. A. After challenge, core body temperature was measured. B. – E. Mice were bled on day 21 after immunization. OVA-specific and total IgG1 and IgE were measured in the serum by ELISA. F. Mice were subjected to passive systemic anaphylaxis. IgE anti-DNP was injected i.p. into mice and then 24h later mice were challenged i.p. with DNP-BSA and core body temperature measured. Data are from three independent experiments with n = 11 mice per group. Data are presented as mean ± SEM. **p<0.01, *p<0.05, unpaired Student’s t test.