Figure 4.

ADAM10^DC−/− mice have impaired T_H2, but intact T_H17 responses. WT and ADAM10^DC−/− mice were sensitized and challenged with intranasal administration of A. alternata extract. A. and B. BALF eosinophils and neutrophils were analyzed by flow cytometry. C. Total medLN cells were stimulated with plate bound anti-CD3ε for 4h with monensin. Intracellular cytokine expression in CD4⁺ T cells was assessed by flow cytometry. Representative contour plots and combined results are shown. D. – F. Total IgG1, IgE, and IgG2b were measured in the serum by ELISA. Data shown is from two independent experiments with n = 5 mice per group. Data are presented as mean ± SEM. **p<0.01, *p<0.05, unpaired Student’s t test.