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Immunohistochemical study for nitrergic neurons in proximal colonic sections. Upper panels: Immunoreactivities for neural NOS (nNOS, red) and FFA3 (green) were identified in the intramuscular nerves and myenteric plexus. Lower panels: The localization of nNOS (green) in neurons was confirmed by the colocalization with a pan-neuronal marker PGP9.5 (red). Colocalization was indicated by arrows (yellow). Bar: 20 μm.
