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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Mol Microbiol. 2017 Nov 28;107(1):94–103. doi: 10.1111/mmi.13870

Figure 2.

Figure 2

Improved motility of the E178A mutant when the D197N allele is co-expressed. Control experiments (indicated) used empty vectors encoding only the needed antibiotic resistance. The plate contained tryptone, 0.26% agar, antibiotics (25 μg/ml each Cm and Km), and 10 μM salicylate to induce expression of the FliP variants. The plate was spotted with 3 μl of overnight cultures and incubated for 7.5 h at 32° C.