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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Am J Transplant. 2017 Sep 8;18(1):74–88. doi: 10.1111/ajt.14434

Figure 2. Immune cell composition in donor blood compared to healthy controls.

Figure 2

Immune cell content in the blood of organ donors and healthy living controls was analyzed by flow cytometry. (A) Left: Frequency of CD4+ and CD8+ T cells, neutrophils and monocytes in peripheral blood of organ donors (n=82, T cells; n=40, monocyte/neutrophil) compared to healthy controls (n=7) expressed as %CD45+ cells (** indicates p<0.001). Right: Graph shows percentage of naïve (CD45RA+CCR7+) and effector-memory (TEM, CD45RA−CCR7−) subsets for CD4+ and CD8+ T cells. N.S.=not significant. (B) Representative flow cytometry plots of lymphocytes in healthy control (top) and deceased organ donors (bottom) showing gating for T, B, CD4+, CD8+ T cells and T cell subset delineation by CD45RA and CCR7 expression. All plots are shown as a function of live (DAPI−), singlet, CD45+ cells. Numbers in quadrants indicate mean ± SEM for entire cohort.