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. 2017 Dec 21;7:18000. doi: 10.1038/s41598-017-18177-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Design and production of the OVX313-containing L2 antigens. (A) Scheme of the PfTrx-L2-OVX313 construct. (B) SDS-PAGE analysis under reducing (+β-mercaptoethanol; +βmet) conditions of the total lysate from IPTG-induced E. coli cells overexpressing the PfTrx-L2-OVX313 antigen (I) and of the flow-through fraction (FT) obtained after heparin chromatography (see Fig. S2 for SDS-PAGE data on additional intermediate samples). The pool of salt-eluted, heparin-purified fractions (HP) electrophoresed under reducing (+βmet) or non-reducing (−βmet) conditions is shown in the last two lanes. Lanes I and FT were cropped from a single gel (see Fig. S7A for a full-length gel image); lanes labeled HP represent the results of single-sample SDS-PAGE analyses separately performed on the post-heparin pool. (C) Size-exclusion chromatography (SEC) analysis (see ‘Methods’ for details) carried out on heparin-purified PfTrx-L2-OVX313; Ve: excluded volume. The corresponding data for the PfTrx-lacking, L2-OVX313 construct are shown in panels D, E (see Fig. S7B for a full-length image of the gel from which lanes I and FT were extracted) and F, respectively.