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. 2017 Dec 21;7:18000. doi: 10.1038/s41598-017-18177-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Atomic Force Microscopy analysis and structure prediction of the PfTrx-L2-OVX313 antigen. Representative topographic AFM image (A) and equivalent disc radius distribution (B) of the PfTrx-L2-OVX313 nanoparticles (see ‘Methods’ for details on AFM analysis and Fig. S3 for the construction of an equivalent radius calibration curve). (C) Predicted structure of the PfTrx-L2-OVX313 fusion protein generated with the SWISS-MODEL 3D structure prediction server and the MODELLER web service using Gallus gallus C4bp and an ancestral thioredoxin (PDB: 4ULX³⁸) as templates.