Fig. 1.

Design and validation of mammalian surface display vector SDGF. a Illustration of the lentivector SDGF. A variant with mCherry substituting GFP was also constructed, called SDRF. b Cells transfected with SDGF and stained with Alexa Fluor 647 labeled anti-C9 antibody. c Cells expressing elafin, Machupo virus glycoprotein (MaCV), or YAP via SDGF were stained with Alexa Fluor 647 labeled elastase (left), or with Alexa Fluor 647 labeled streptavidin plus either biotinylated transferrin receptor ectodomain (TfR, middle) or biotinylated YAP-binding domain of TEAD (right). d Cells expressing SDGF-elafin were mixed with cells expressing SDRF-MaCV at approximately a 1:500 dilution. The cells were stained with Alexa Fluor 647 labeled elastase, and were flow sorted by Alexa Fluor 647 content (left) with a generous gate to collect all labeled cells. The resulting proportion of green (SDGF) cells increased from 0.2% pre-sort (middle) to 17.9% post-sort (right)