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. 2017 Dec 21;8:2249. doi: 10.1038/s41467-017-02353-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Cell cycle delay after CELF1 depletion in melanoma cells. a Efficiency of lentiviral-based transfer of CELF1 shRNAs (shCELF1-1 and 2) in the indicated cell lines visualized by immunoblotting with respect to control shRNA. b Impaired growth of SK-Mel-103 and UACC-62 cells upon CELF1 depletion. Time points represent days after lentiviral infection. Error bars correspond to SEM of three independent experiments in triplicate. c Cell cycle profiles of shControl and shCELF1 transduced SK-Mel-103 cells at the indicated times after release from thymidine block. d Quantification of data in c. e Melanoma cells expressing shControl or shCELF1 (upper panel) analyzed for colony formation capability (crystal violet staining) 12 days after cell seeding. f Quantification of inhibited colony formation of cells as in e. Data are represented as the ratio of inhibition with respect to shControl transduced cells (for simplicity here shown as a gray bar only for SK-Mel-5). Error bars correspond to SEM of two independent experiments in duplicates