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. 2017 Dec 21;7:17962. doi: 10.1038/s41598-017-17320-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Endosomally located phosphorylation deficient Eps15-GFP and Hrs-YFP are unaffected by ligand stimulation. (A) montage showing EGF-Alexa-647 internalization and localization to enlarged Eps15Y850F-GFP endosomes. (B) A montage showing EGF-Alexa-647 internalization and localization to enlarged HrsY329/334F-YFP endosomes. (c) This graph shows the endosomal fluorescence intensity of the Eps15 tyrosine mutant (Eps15Y850F-GFP) after stimulation with unlabelled EGF/PDGF. Data represents the mean value of 10 independent experiments ± s.d. (d) This graph shows the endosomal fluorescence intensity of the of the Hrs-YFP tyrosine mutant (HrsY329, 334F-YFP) after stimulation with unlabelled EGF/PDGF. Data represents the mean value of 10 independent experiments ± s.d.