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. 2017 Dec 21;7:17962. doi: 10.1038/s41598-017-17320-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The mutants Eps15Y850F-GFP and HrsY329/334F-YFP reduce the EGF-R and PDGF-R degradation. The figure shows an EGF-R degradation assay on M1 cells stably expressing Ii-pMep4, co-transfected with either (a) Eps15-GFP, (b) Hrs-YFP, (c) Eps15Y850F-GFP or (d) HrsY329/334F-YFP. The cells were treated with cycloheximide (CHX) and stimulated with unlabelled EGF or PDGF BB. The amount of EGF-R was analysed with an antibody against EGF-R, and the total amount of proteins present on the membrane was detected with antibodies against Eps15/Hrs/GFP. Quantification of EGF-R (e) and PGDF-R (f) degradation assay are based on three individual experiments.