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. 2017 Dec 21;7:18022. doi: 10.1038/s41598-017-18364-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Study design and flight/ground nanopore performance. (A) A mixture of equimolar DNA from mouse, E. coli and lambda phage genomes was sequenced in parallel on Earth (“Ground”) and in-flight on the ISS (after being delivered by a SpaceX Dragon capsule; flow cells were shipped to the Kennedy Space Center on July 11, 2016). Synchronous nanopore sequencing runs were performed from August 26, 2016 to January 9, 2017. (B) Plot of mean current intensity in picoAmperes (pA; Y-axis) against k-mers (x-axis) in order of increasing mean current based on a model distribution from Oxford Nanopore Technologies (black). Current distributions are tightly clustered with the exception of lower-quality ground #2. (C) Comparison of pairwise identities of aligned reads between ISS runs 1–4 and ground runs 1–4. (D) Pie charts of the read distributions corresponding to each ISS run and pooled ISS runs 1–4.