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. 2017 Dec 21;7:18041. doi: 10.1038/s41598-017-18029-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Candidate sites of Uox for site-specific incorporation of AzF. (a) Structures of six Uox variants (Uox-E22, Uox-K23, Uox-D112, Uox-K138, Uox-W160, and Uox-Q243). The crystal structure of Uox-WT (PDB ID: 1WS2) was viewed by the PyMOL program. The AzF incorporation site and active site are marked in blue and red, respectively. (b) Solvent accessibilities of site candidates for AzF incorporation, predicted by ASA-View Program. Red dot-line indicates the solvent accessibility value of 0.5.