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. 2017 Dec 21;7:17964. doi: 10.1038/s41598-017-17445-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree based on 16S rRNA gene sequences for the 19 MTB OTUs identified from live MTB samples. The phylogenetic tree was constructed based on neighbor-joining analysis. Bootstrap values at the nodes are percentages of 1,000 replicates. We used 97% and 95% sequence identity as the thresholds for classifying to species and genus levels, respectively. OTUs belonging to new species are indicated in blue. OTUs belonging to new genera are indicated in red. OTUs identified from micromanipulated samples are emphasized in bold characters. The scale bar indicates 2% sequence divergence.