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. 2017 Dec 21;7:18026. doi: 10.1038/s41598-017-18168-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plasmids cloned into ung ⁻ cells contain undetectable levels of uracil. (A) Experimental scheme used. (B) Gel of plasmids isolated from ung ⁺ cells transformed with a thymine containing insert (black – lanes 2, 5–8) or ung ⁻ cells transformed with dTTP (green – 3, 9–12) or dUTP (blue – 4, 13–16). The samples remained untreated (lanes 2–4) or were incubated with 0.5U of Ung (lanes 5–16). Controls for Ung digestion consist of SUMO1 cDNA amplicons containing uracil digested with Ung (lane 17) or left untreated (lane 18). MW = molecular weight markers (lanes 1, 19). The numbers above the lanes refer to the name of the clones tested.