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. 2017 Dec 21;7:17978. doi: 10.1038/s41598-017-18210-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Androgen receptor (AR) binds to AURKA. (a) AR binding to the KLK3-enhancer (n = 1), (b) AURKA-promoter and (c) intronic region under DHT stimulation (n = 2). Traditional AR-ChIP-qPCR was performed after hormone starvation for 4 days followed by treatment with 1 and 100 nM DHT stimulation of LNCaP-pcDNA3.1 (control) and two independent AR-overexpressing cell lines (LNCaP-ARmo and LNCaP-ARhi). Bars show enrichment of AR binding expressed as the % of input. *P < 0.0232 and **P < 0.0107, unpaired t test.