Fig. 8. NOX2-deficient bone marrow transplantation attenuates high-fat diet-induced hepatic steatosis in mice.

After reciprocal bone marrow transplantation between WT and Nox2 KO mice, mice were fed a high-fat diet for 12 weeks. a GTT was performed after 16 h fasting. b Liver gross findings and hepatic contents of TG were assessed. c Liver sections were stained with H&E (upper panels) and PAS (lower panels). Bar = 100 μm. d Isolated liver MNCs were subjected to qRT–PCR analyses. e Whole liver tissues were subjected to Western blottings. f Schematic diagram of NOX2-mediated ROS generation through endocytosis of monomeric TLR4–MD2 complex in non-resident macrophages by palmitate and its involvement in the pathogenesis of hepatic steatosis and insulin resistance. Data are representative of two independent experiments using 5 mice per group. Data are expressed as the mean ± s.e.m. and analyzed by one-way analysis of variance, *P < 0.05 in comparison with the corresponding controls