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. 2017 Nov 29;175(1):38–52. doi: 10.1111/bph.14069

Figure 3.

Figure 3

LU‐005i inhibits the production of inflammatory cytokines. (A–C) Splenocytes derived from C57BL/6 (A–C) or LMP7−/− (A) mice were exposed to the indicated concentrations of LU‐005i (A, C) or LU‐001i (B, C) and stimulated with LPS (A, B) or plate‐bound antibodies specific for CD3/CD28 (C) overnight. IL‐6 (A, B) or IFN‐γ (C) in the supernatant was analysed by elisa. Each data point was measured in triplicates. The highest cytokine concentration from each experiment was set to 100% to control for unwanted variations in cytokine secretion between individual experiments. Data are presented as the mean ± SEM of six (A, B) or five (C) independent experiments. (D, E) Human PBMCs were exposed to the indicated concentrations of LU‐005i (left side) or LU‐001i (right side) and stimulated with LPS overnight. IL‐6 (D) or IL‐23 (E) in the supernatant was analysed by elisa. Each data point was measured in triplicates. The highest cytokine concentration from each experiment was set to 100% to control for unwanted variations between individual donors. Data are presented as the mean ± SEM of six (D) or eight (E) independent donors. All data were statistically compared to the DMSO treated group. *P < 0.05.