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. 2017 Nov 29;175(1):38–52. doi: 10.1111/bph.14069

Figure 4.

Figure 4

LU‐005i blocks Th17 differentiation. Differentiation of CD4+ T cells from C57BL/6 (A, C) or LMP7−/− (B) mice exposed to the indicated concentrations of LU‐005i (A, B) or LU‐001i (C) and stimulated with antibodies to CD3/CD28 in the presence of TGF‐β and IL‐6 and antibodies to IL‐4 and IFN‐γ was measured in 3 day cultures. IL‐17 expression was detected by intracellular cytokine staining after a short restimulation with PMA/ionomycin. IL‐17 expression in activated T cells cultured in the absence of Th17‐polarizing conditions is shown as a comparison (no cocktail). Pooled data from five independent experiments done in triplicates are shown as means ± SEM. Values reflect the percentage of CD4+ cells that are also IL‐17A+. All data were statistically compared to the DMSO‐treated group. *P < 0.05. The ‘no cocktail’ group was excluded from statistical analysis due to variance inhomogeneity.