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. 2017 Dec 8;175(1):125–139. doi: 10.1111/bph.14078

Figure 4.

Figure 4

Effect of IGF‐1 on the activity of intracellular ROS and MDA in amiodarone (AM)‐treated D407 cells. (A–C) After pretreatment with IGF‐1(100 ng·mL−1) for 1 h, D407 cells were incubated with or without 50 μM amiodarone for another 24 h then stained with DCFH‐DA and analysed by fluorometry to detect ROS, or by the application of MDA detection kit to measure MDA; data are presented as means ± SEM of the results obtained from five experiments. #P < 0.05 versus control, *P < 0.05 versus amiodarone‐treated group.