Figure 5.
IGF‐1 attenuated amiodarone (AM)‐induced loss of mitochondrial membrane potential (△ψm) loss and increase in caspase 3/7 activity in D407 cells. (A, B) After pretreatment with IGF‐1(100 ng·mL−1) for 1 h, D407 cells were incubated with or without 50 μM amiodarone for another 6 h. The △ψm was determined by the JC‐1 assay. (C) Quantification of caspase 3/7 activity was determined by caspase 3/7 activity assay. Data are presented as means ± SEM of the results obtained from five to six experiments. #P < 0.05 versus control group; *P < 0.05 versus amiodarone‐treated group.