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Effects of amiodarone (AM) on phosphorylation of the Akt and ERK1/2. (A, B) D407 cells were treated with 50 μM amiodarone for various times or with different concentrations of amiodarone for 24 h, and then, the phosphorylation of Akt and MAPK (ERK1/2) in D407 cells was determined as described in Methods. Blots and quantitative data (OD) are shown (C–F). Data are presented as means ± SEM of the results obtained from five experiments. *P < 0.05 versus control.
