Figure 3.

Human and mouse CXCL17 responses as determined by the AP‐TGF‐α shedding assay. (A) Effects of human and mouse CXCL17 in the AP‐TGFα shedding assay. Release of AP‐TGFα by both human and mouse CXCL17 was measured in HEK‐293 cells transfected with GPR35 and a mixture of eight Gα proteins. Results are presented as the means ± SEM of three individual experiments. Concentration–response curve for lodoxamide was duplicated from Figure 1 for a comparison. (B) HEK‐293 cells were transfected with plasmids of human GPR35 or mock plasmid along with AP‐TGF‐α and G proteins 24 h, and then, GPR35 proteins (B) were evaluated by Western blotting.