TABLE 2.
Antimicrobial susceptibility profiles of six clinical isolates, an E. coli J53A transconjugant of AMA 754 (AMA 1292), and E. coli J53A and β-lactam susceptibilities of E. coli TOP10 expressing β-lactamases and E. coli TOP10
| Antibiotic | MIC (mg/liter) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| E. asburiae AMA 497 | E. asburiae AMA 590 | C. freundii AMA 754 | K. pneumoniae AMA 782 | E. asburiae AMA 810 | C. freundii AMA 948 | E. coli AMA 1292a | E. coli J53A3R | E. coli TOP10(pCR-BluntII-OXA-436) | E. coli TOP10(pCR-BluntII-OXA-48) | E. coli TOP10(pCR-BluntII-OXA-181) | E. coli TOP10 | |
| Temocillin | >256 | >256 | >256 | >256 | >256 | >256 | >256 | 8 | >256 | >256 | >256 | 16 |
| Piperacillin-tazobactam | >64 | >64 | >64 | >64 | >64 | >64 | >64 | 2 | >64 | >64 | >64 | 2 |
| Ceftazidime | >32 | >32 | 32 | >32 | >32 | >32 | >32 | 0.25 | 0.5 | 1 | 1 | 0.5 |
| Ceftazidime-avibactam | 0.5 | 0.25 | 0.5 | 0.5 | 0.25 | 1 | 0.5 | 0.125 | 0.25 | 0.5 | 1 | 0.25 |
| Cefotaxime | 8 | 8 | 8 | 4 | 8 | >16 | 4 | ≤0.06 | 0.125 | 1 | 2 | 0.125 |
| Cefoxitin | >32 | >32 | >32 | 16 | >32 | >32 | 16 | 8 | 16 | 16 | 32 | 4 |
| Aztreonam | >16 | >16 | >16 | >16 | >16 | >16 | >16 | ≤0.125 | ≤0.125 | 0.25 | 0.25 | ≤0.125 |
| Meropenem | 4 | 4 | 1 | 2 | 4 | 1 | 1 | 0.032 | 0.5 | 1 | 1 | 0.032 |
| Imipenem | 4 | 4 | 2 | 2 | 4 | 2 | 2 | 0.25 | 2 | 2 | 2 | 0.25 |
| Ertapenem | >4 | 4 | 4 | >4 | >4 | 4 | 4 | ≤0.015 | 1 | 2 | 2 | ≤0.015 |
| Gentamicin | 128 | 128 | 64 | 128 | 64 | 64 | 32 | ≤0.25 | NDb | ND | ND | ND |
| Amikacin | <1 | <1 | <1 | <1 | <1 | <1 | <1 | <1 | ND | ND | ND | ND |
| Tobramycin | 4 | 8 | 8 | 8 | 8 | 4 | 2 | <0.5 | ND | ND | ND | ND |
| Ciprofloxacin | 4 | 4 | 8 | 16 | 4 | 2 | 0.5 | <0.015 | ND | ND | ND | ND |
| Tigecycline | 0.5 | 0.5 | 2 | 1 | 1 | 0.5 | 0.25 | 0.25 | ND | ND | ND | ND |
| Colistin | 0.5 | 0.5 | 1 | 0.5 | 0.5 | 1 | ≤0.25 | 0.5 | ND | ND | ND | ND |
| Trimethoprim-sulfamethoxazole | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | ND | ND | ND | ND |
a
AMA 1292 is the E. coli J53A transconjugant of AMA 754.
b
ND, not determined.