TABLE 3.

Kinetic parameters of OXA-436 compared to OXA-48 and OXA-181

Subtrate	Km (μM)			kcat (s−1)			kcat/Km (μM−1 · s−1)
	OXA-436	OXA-48a	OXA-181b	OXA-436	OXA-48a	OXA-181b	OXA-436	OXA-48a	OXA-181b
Benzylpenicillin	200 ± 100	79	90	900 ± 100	446	444	4.5	5.6	5.0
Ampicillin	5 ± 1	395	170	600 ± 40	955	218	120	2.4	1.3
Temocillin	200 ± 100	45	60	3 ± 1	0.3	0.3	0.015	0.0066	0.005
Cefotaxime	130 ± 20	190	>1000	6 ± 0.4	10	>62	0.046	0.06	0.013
Ceftazidime	150 ± 50	5100	NHc	7 ± 1	4	NDd	0.047	0.001	ND
Imipenem	20 ± 1	13	13	6 ± 0.2	4.8	7.5	0.3	0.37	0.55
Meropenem	3 ± 1	11	70	0.14 ± 0.01	0.07	0.1	0.047	0.0062	0.0015
Ertapenem	160 ± 60	100	100	0.4 ± 0.05	0.13	0.2	0.0025	0.0013	0.002

^aKinetic parameters for OXA-48 are from Docquier et al. (28), except for those for benzylpenicillin, which are from Antonelli et al. (29), and cefotaxime-ceftazidime, which are from Poirel et al. (3).

^bKinetic parameters for OXA-181 are from Oueslati et al. (12).

^cNH, no detectable hydrolysis.

^dND, not determined.