TABLE 3.
MICs of fluconazole and tebuconazole for non-TBZ-adapted C. neoformans colonies, C. neoformans colonies subjected to TBZ adaptation at 30°C, and TBZ-adapted C. neoformans colonies subcultured 10 times in agrochemical-free mediuma
| Strain | MIC (μg/ml) at indicated temp (geometric mean)b |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fluconazole |
Tebuconazole |
|||||||||||
| 30°C |
35°C |
30°C |
35°C |
|||||||||
| NA | A | 10p | NA | A | 10p | NA | A | 10p | NA | A | 10p | |
| H99 | 16.0 | 128.0 [8×] | 32.0 | 8.0 | 64.0 [8×] | 8.0 | 1.0 | 4.0 [4×] | 1.0 | 0.25 | 2.0 [8×] | 0.25 |
| ATCC 24067 | 16.0 | 64.0 [4×] | 16.0 | 4.0 | 32.0 [8×] | 4.0 | 0.5 | 2.0 [4×] | 0.5 | 0.25 | 1.0 [4×] | 0.25 |
| ATCC 28957 | 4.0 | 16.0 [4×] | 32.0 [8×] | 2.0 | 4.0 | ND | 0.5 | 4.0 [8×] | 2.0 [4×] | 0.5 | 0.5 | ND |
| ATCC 62066 | 4.0 | 16.0 [4×] | 4.0 | 4.0 | 4.0 | ND | 0.25 | 1.0 [4×] | 0.5 | 0.5 | 0.5 | ND |
| Range | 4.0–16.0 (8.0) | 16.0–128.0 (38.05) | ND (ND) | 2.0–8.0 (4.0) | 4.0–64.0 (13.45) | ND (ND) | 0.25–1.0 (0.5) | 1.0–4.0 (2.38) | ND (ND) | 0.25–0.5 (0.35) | 0.5–2.0 (0.84) | ND (ND) |
a
Tests were performed at 30°C and 35°C.
b
MIC values represent endpoint values (MIC at 50% of growth inhibition). Numbers in square brackets indicate how many times (×) higher the drug MIC value for the TBZ-adapted colonies (A) or colonies subjected to 10 passages (10p) was (≥4×) than the drug MIC value for the non-TBZ-adapted (NA) colonies. Values highlighted in bold indicate MIC values that were at least 4× higher than those seen with the NA colonies. ND, not determined.