TABLE 8.
MICs of itraconazole and ravuconazole for non-TBZ-adapted C. gattii and C. neoformans colonies, C. gattii and C. neoformans colonies subjected to TBZ adaptation at 35°C, and TBZ-adapted C. gattii and C. neoformans colonies subcultured 10 times in agrochemical-free mediuma
| Strain | MIC (μg/ml) (geometric mean)b |
|||||
|---|---|---|---|---|---|---|
| Itraconazole |
Ravuconazole |
|||||
| NA | A | 10p | NA | A | 10p | |
| C. gattii | ||||||
| R265 | 0.25 | 0.25 | 0.25 | 0.03 | 0.5 [16×] | ND |
| 196L/03 | 0.5 | 1.0 | 1.0 | 0.125 | 1.0 [8×] | 1.0 [8×] |
| 23/10893 | 0.5 | 0.5 | ND | 0.03 | 0.06 | ND |
| C. neoformans | ||||||
| H99 | 0.25 | 2.0 [8×] | ND | 0.03 | 0.5 [16×] | ND |
a
Tests were performed at 35°C.
b
MIC values represent endpoint values (MIC at 50% of growth inhibition). Numbers in square brackets indicate how many times (×) higher the drug MIC value for the TBZ-adapted colonies (A) or colonies subjected to 10 passages (10p) was (≥4×) than the drug MIC value for the non-TBZ-adapted (NA) colonies. Values highlighted in bold indicate MIC values that were at least 4× higher than those seen with the NA colonies. ND, not determined.