FIG 3.

Agar CHX susceptibility assay. The geometric means and the geometric standard deviations of the CFU/ml are shown for wild-type E. faecium 410 and the ΔefrEF deletion mutant (A), the DAP-A2 mutant and the DAP-A2 ΔefrEF deletion mutant (PB302) (B), and the ΔefrEF deletion mutant transformed with empty pLZ12 vector or the complementation vectors pAH201 or pPB202 (C). The CFU/ml value was determined using BHI or BHI-chloramphenicol agars with or without CHX supplementation at 1/8×, 1/4×, or 1/2× MIC CHX. Data from three independent trials are shown for each condition. The dashed line represents the limit of detection (10³ CFU/ml). *, P < 0.05 value (one-tailed Student t test). Cam, chloramphenicol.