TABLE 3.
Mutations identified by whole-genome sequencing in the DAP-resistant mutants
| Mutant | Gene description | Nucleotide variation (frequency [%])a | Amino acid changeb | E. faecalis V583 ortholog |
|---|---|---|---|---|
| DAP-A1 | Eftg_02287 ABC transporter | C67008T (98.47) | Ala290Val | EF2226 |
| Eftg_01724 RelA/SpoT (relA) | CTAGGATTTAC30479Del (98.19) | Leu505fs | EF1974 | |
| Eftg_01135 alpha/beta hydrolase | G137748A (55.64) | Trp150* | EF1505 | |
| Eftg_00577 cytidylate kinase (cmk) promoter | A618699IS1251 | IS1251 insertion | EF1547 | |
| DAP-A2 | Eftg_02287 ABC transporter | C67008T (99.77) | Ala290Val | EF2226 |
| Eftg_01724 RelA/SpoT (relA) | CA29139AG (95.74) | Ala58Glu | EF1974 | |
| Eftg_01135 alpha/beta hydrolase | 137847G insertion (88.17) | Ile184fs | EF1505 | |
| Eftg_00577 cytidylate kinase (cmk) promoter | A618699IS1251 | IS1251 insertion | EF1547 | |
| DAP-B1 | Eftg_02287 ABC transporter | C67008T (98.962) | Ala290Val | EF2226 |
| Eftg_01175 PhoU regulator | G173845A (99.61) | Glu61Lys | EF1754 | |
| Eftg_02534 transposase | C71844A (99.47) | Lys206Asn | ||
| DAP-B2 | Eftg_02287 ABC transporter | C67008T (99.36) | Ala290Val | EF2226 |
| Eftg_02162 glycosyltransferase (bgsB) | C50223T (67.09) | Val57Met | EF2890 |
a
The mutation frequency in the read assembly was determined by variant detection in CLC Genomics Workbench where applicable. The precise location of insertion for variation A618699IS1251 was mapped by Sanger sequencing.
b
Asterisk indicates stop codon.