FIG 1.

Effect of miltefosine and sphingosine on the intracellular Ca²⁺ concentration of L. donovani promastigotes. Promastigotes were loaded with Fura-2 and the indicated compounds were added directly to the cuvette, as described in Materials and Methods. (A) Miltefosine (4 μM) was added (arrow) in the presence of 2 mM extracellular Ca²⁺, followed by the addition of sphingosine (10 μM). (B) Sphingosine (10 μM) was added as indicated (arrow), followed by miltefosine (4 μM). Traces are representative of at least three independent experiments.