FIG 2.

Effect of miltefosine and the Ca²⁺ channel agonist Bay K 8644 on the intracellular Ca²⁺ concentration of L. donovani promastigotes. (A) Miltefosine (4 μM) and then Bay K 8644 (4 μM) were added (arrows) directly to the cuvette in the presence of 2 mM extracellular Ca²⁺. (B) Bay K 8644 (4 μM) was added (arrow), followed by miltefosine (4 μM) when indicated, in the presence of 2 mM extracellular Ca²⁺. Traces are representative of at least three independent experiments. (See Materials and Methods section for details).