FIG 4.

Effect of miltefosine on the mitochondrial electrochemical potential of L. donovani promastigotes. Parasites were incubated in the presence of rhodamine123 (10 mg/ml) for 30 min at room temperature, as indicated in Materials and Methods. (A) In the presence of 2 mM extracellular Ca²⁺, miltefosine (4 μM) was added (arrow), followed by FCCP (2 μM). (B) Miltefosine (4 μM) was added (arrow), followed by addition of FCCP (2 μM). This was performed in the absence of extracellular Ca²⁺. Traces are representative of at least three independent experiments.