FIG 5.

Effect of miltefosine on acidocalcisomes in L. donovani promastigotes. Parasites were loaded with acridine orange (2 mM) as described in Materials and Methods. The excitation wavelength was 488 nm, and emission was at 530 nm. (A) Miltefosine (4 μM) was added (arrow) directly to the stirring cuvette with promastigotes loaded with acridine orange, followed by the addition of nigericin (2 μM). This was performed in the absence of extracellular Ca²⁺. (B) In the absence of extracellular Ca²⁺, nigericin was added at 2 μM (arrow), followed by miltefosine (4 μM). Traces are representative of at least three independent experiments.