TABLE 1.
Organism | Strain | MIC (μg/ml)f |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
CFDC | CAZ | CFPM | MEPM | PIPC-TAZ | CAZ-AVI | CFT-TAZ | COL | AMK | CPFX | ||
Gram-negative bacteria | |||||||||||
Acinetobacter baumannii | ATCC 19606 | 0.063 | 8 | 16 | 1 | 16 | 16 | 2 | 0.5 | 16 | 1 |
Acinetobacter calcoaceticus | ATCC 23055 | 0.063 | 0.125 | 0.063 | ≤0.031 | ≤0.031 | 0.063 | ≤0.031 | 0.25 | 0.25 | ≤0.031 |
Acinetobacter haemolyticus | ATCC 17906 | ≤0.031 | 2 | 1 | 0.25 | ≤0.031 | 4 | ≤0.031 | 1 | >32 | 0.125 |
Acinetobacter johnsonii | ATCC 17909 | 0.125 | 8 | 2 | 0.25 | ≤0.031 | 16 | ≤0.031 | 0.25 | 1 | 0.25 |
Acinetobacter lwoffii | ATCC 15309 | ≤0.031 | 1 | 0.25 | 0.063 | ≤0.031 | 2 | ≤0.031 | 0.125 | 0.5 | 0.25 |
Aeromonas hydrophila | IFO3820 | 0.125 | 0.5 | 0.063 | 2 | 4 | 0.25 | 1 | >32 | 2 | ≤0.031 |
Bordetella parapertussis | NCTC 5952 | 1 | 1 | 1 | ≤0.031 | ≤0.031 | 0.5 | 1 | ≤0.031 | 2 | ≤0.031 |
Burkholderia cepacia | ATCC 25416 | ≤0.031 | 4 | 32 | 4 | 32 | 2 | 2 | >32 | >32 | 1 |
Burkholderia multivorans | SR01869 | 2 | 2 | 32 | 4 | >32 | 2 | 4 | >32 | >32 | 4 |
Campylobacter jejuni | ATCC 33560 | >4 | >16 | 2 | 0.015 | 64 | 4 | NT | NT | NT | 0.25 |
Campylobacter jejuni | 794009 | >4 | >16 | 2 | 0.015 | 64 | 4 | NT | NT | NT | >4 |
Citrobacter freundii | ATCC 8090 | 0.063 | 1 | ≤0.031 | ≤0.031 | 1 | 0.125 | 0.25 | 0.5 | 2 | ≤0.031 |
Elizabethkingia meningoseptica | NCTC 10016 | 1 | >32 | >32 | 16 | >32 | >32 | >32 | >32 | 32 | 8 |
Enterobacter aerogenes | ATCC 13048 | ≤0.031 | 1 | 0.063 | 0.063 | 4 | 0.5 | 0.5 | 0.25 | 2 | ≤0.031 |
Enterobacter cloacae | ATCC 13047 | 0.5 | 8 | 0.125 | 0.063 | 16 | 0.5 | 8 | >32 | 2 | ≤0.031 |
Enterobacter cloacaea | NCTC 13464 | 0.125 | 2 | 8 | 0.063 | 2 | 0.25 | 0.5 | 0.25 | 2 | ≤0.031 |
Escherichia coli | ATCC 25922 | 0.125 | 0.5 | 0.125 | ≤0.031 | 2 | 0.5 | 0.25 | 0.5 | 1 | ≤0.031 |
Escherichia coli | ATCC 35218 | ≤0.031 | 0.25 | 0.125 | ≤0.031 | 1 | 0.125 | 0.25 | 0.5 | 2 | ≤0.031 |
Escherichia colia | NCTC 13462 | 2 | 8 | >32 | ≤0.031 | 2 | 1 | 2 | 0.25 | 4 | 0.25 |
Haemophilus influenzae | ATCC 10211 | 0.25 | 0.063 | ≤0.031 | 0.063 | ≤0.031 | ≤0.031 | 0.125 | 0.25 | 8 | ≤0.031 |
Haemophilus influenzae | ATCC 49247 | 2 | 0.5 | 1 | 0.125 | 0.125 | 0.125 | 1 | 0.25 | 8 | ≤0.031 |
Haemophilus parainfluenzae | ATCC 7901 | 0.25 | ≤0.031 | ≤0.031 | ≤0.031 | ≤0.031 | ≤0.031 | ≤0.031 | 0.25 | 1 | ≤0.031 |
Klebsiella oxytoca | ATCC 13182 | 0.063 | 0.25 | 0.125 | 0.063 | 4 | 0.5 | 0.5 | 0.25 | 2 | 0.063 |
Klebsiella pneumoniae | ATCC 43816 | ≤0.031 | 0.125 | 0.063 | 0.063 | 2 | 0.25 | 0.25 | 0.25 | 1 | 0.063 |
Moraxella catarrhalis | ATCC 25238 | ≤0.031 | 0.063 | 0.125 | ≤0.031 | ≤0.031 | 0.063 | ≤0.031 | 1 | 0.5 | ≤0.031 |
Morganella morgannii | ATCC 25830 | ≤0.031 | 0.125 | 0.063 | 0.125 | ≤0.031 | 0.063 | 0.125 | >32 | 1 | ≤0.031 |
Neisseria gonorrhoeae | ATCC 49226 | 0.5 | 0.12 | 0.12 | 0.03 | ≤0.25 | 0.12 | NT | NT | NT | 0.004 |
Neisseria gonorrhoeaeb | 867807 | 0.25 | 0.12 | 0.06 | 0.015 | ≤0.25 | 0.12 | NT | NT | NT | 0.008 |
Neisseria gonorrhoeaec | 868339 | >4 | >16 | >8 | 0.06 | 1 | >16 | NT | NT | NT | >4 |
Neisseria meningitidis | ATCC 13077 | 0.125 | 0.063 | ≤0.031 | ≤0.031 | ≤0.031 | ≤0.031 | ≤0.031 | >32 | 8 | ≤0.031 |
Proteus mirabilis | ATCC 29906 | ≤0.031 | 0.25 | 0.125 | 0.125 | 0.5 | 0.125 | 0.5 | >32 | 4 | ≤0.031 |
Proteus vulgaris | ATCC 13315 | ≤0.031 | 0.125 | 0.063 | 0.125 | ≤0.031 | 0.063 | 0.25 | 32 | 0.5 | ≤0.031 |
Providencia alcalifaciens | ATCC 9886 | ≤0.031 | 0.25 | 0.063 | 0.063 | 2 | 0.25 | 0.125 | >32 | 4 | ≤0.031 |
Providencia rettgeri | ATCC 29944 | ≤0.031 | 0.25 | 1 | 0.063 | 4 | 0.25 | >32 | >32 | 0.5 | ≤0.031 |
Providencia stuartii | ATCC 29914 | ≤0.031 | 0.25 | 0.063 | 0.125 | 1 | 0.5 | 0.25 | >32 | 0.5 | ≤0.031 |
Pseudomonas aeruginosa | ATCC 27853 | 0.5 | 2 | 2 | 0.25 | 2 | 2 | 0.5 | 0.5 | 4 | 0.25 |
Pseudomonas aeruginosad | NCTC 13437 | 0.5 | >32 | >32 | >32 | >32 | >32 | >32 | 1 | 32 | 32 |
Pseudomonas putida | ATCC 12633 | 0.125 | 2 | 2 | 1 | 8 | 2 | 0.5 | 0.5 | 0.5 | 0.063 |
Pseudomonas stutzerii | ATCC 11607 | 0.125 | 0.5 | 0.125 | 0.125 | 2 | 0.5 | 0.125 | 0.125 | 0.5 | ≤0.031 |
Salmonella enterica serovar Choleraesuis | ATCC 51741 | 0.015 | 0.5 | 0.06 | 0.03 | 4 | 0.5 | NT | NT | NT | 0.03 |
Salmonella enterica serovar Enteritidis | G-14 | 0.25 | 1 | 0.063 | ≤0.031 | 2 | 0.5 | 1 | 1 | 16 | ≤0.031 |
Salmonella enterica serovar Paratyphi | 598989 | 0.06 | 0.5 | 0.06 | 0.03 | 4 | 0.25 | NT | NT | NT | >4 |
Salmonella enterica serovar Typhi | 673937 | 0.015 | 0.5 | 0.12 | 0.03 | 2 | 0.25 | NT | NT | NT | >4 |
Salmonella enterica serovar Typhimurium | ATCC 13311 | ≤0.031 | 0.5 | 0.063 | ≤0.031 | 4 | 0.5 | 0.5 | 0.5 | 2 | ≤0.031 |
Serratia marcescens | ATCC 13880 | ≤0.031 | 0.5 | 0.25 | 0.063 | 2 | 1 | 0.5 | >32 | 1 | 0.063 |
Shigella flexnerie | 705927 | 0.5 | 0.06 | 0.25 | 0.03 | 2 | 0.06 | NT | NT | NT | 0.015 |
Stenotrophomonas maltophilia | ATCC 13637 | 0.125 | 32 | >32 | >32 | >32 | 32 | 32 | 1 | 4 | 0.25 |
Vibrio fluvialis | NCTC 11327 | 0.25 | 0.25 | 0.25 | 0.25 | 4 | 0.5 | 4 | 0.25 | 2 | ≤0.031 |
Vibrio vulnificus | ATCC 27562 | 1 | 0.5 | 1 | ≤0.031 | 0.063 | ≤0.031 | 2 | 4 | 2 | ≤0.031 |
Yersinia enterocolitica | ATCC 9610 | ≤0.031 | 0.125 | 0.063 | 0.063 | 1 | 0.125 | 0.25 | 0.25 | 2 | 0.063 |
Yersinia pseudotuberculosis | ATCC 29833 | 0.5 | 0.25 | 0.063 | ≤0.031 | 0.5 | 0.25 | 0.25 | >32 | 2 | ≤0.031 |
Gram-positive bacteria | |||||||||||
Bacillus subtilis | ATCC 6633 | 32 | 4 | 1 | 0.063 | 0.25 | 4 | 2 | 8 | 0.25 | ≤0.031 |
Enterococcus faecalis | ATCC 29212 | >32 | >32 | 16 | 2 | 2 | >32 | 32 | >32 | >32 | 1 |
Lactobacillus casei | ATCC 393 | >32 | 4 | 32 | 1 | 0.5 | 4 | 2 | >32 | 2 | 1 |
Micrococcus luteus | ATCC 9341 | 4 | 0.5 | ≤0.031 | 0.063 | ≤0.031 | 0.5 | 0.5 | >32 | 1 | 2 |
Staphylococcus aureus | ATCC 29213 | 32 | 8 | 8 | 0.125 | 1 | 8 | 32 | >32 | 2 | 0.5 |
Streptococcus pneumoniae | ATCC 49619 | 2 | 0.5 | ≤0.031 | 0.063 | 0.5 | 0.5 | 0.25 | >32 | 32 | 0.5 |
Streptococcus pyogenes | ATCC 10389 | 1 | 0.125 | ≤0.031 | ≤0.031 | 0.063 | 0.125 | 0.125 | >32 | 16 | 0.125 |
CTX-M type β-lactamase producer.
Resistant to azithromycin.
Resistant to ceftriaxone.
VIM-10 and VEB-1 β-lactamase producer.
Resistant to tetracycline and trimethoprim-sulfamethoxazole.
MICs of cefiderocol (CFDC) were determined in iron-depleted cation-adjusted Mueller Hinton broth (ID-CAMHB), and those of other antibiotics were determined in CAMHB except when the following was used (see supplemental method 2): (i) CAMHB supplemented with 2.5 to 5.0% lysed horse blood for B. parapertussis, Streptococcus pneumoniae, Streptococcus pyogenes, Campylobacter jejuni, Neisseria meningitidis, and Lactobacillus casei, (ii) Haemophilus test medium broth for Haemophilus spp., or (iii) GC agar supplemented with 1% defined growth supplement for Neisseria gonorrhoeae, except for meropenem, which was determined on GC agar supplemented with 1% defined growth supplement without cysteine component. NT, not tested; CFDC, cefiderocol; CAZ, ceftazidime; CFPM, cefepime; MEPM, meropenem; PIPC-TAZ, piperacillin-tazobactam; CAZ-AVI, ceftazidime-avibactam; CFT-TAZ, ceftolozane-tazobactam; COL, colistin; AMK, amikacin; CPFX, ciprofloxacin. TAZ and AVI were at a fixed concentration of 4 μg/ml. MICs of PIPC-TAZ, CAZ-AVI, and CFT-TAZ are represented as the concentrations of PIPC, CAZ, and CFT, respectively.