Figure 5.

UFH inhibits activation of the p38 MAPK, Src and NF-κB signaling pathways in LPS-stimulated human umbilical vein endothelial cells. A total of 6 h after LPS treatment, the phosphorylation status of (A) p38 MAPK, (B) Src, (C) IκBα, and (D) nuclear and cytosolic NF-κB were detected by western blot analysis. β-actin was used as the internal control for total protein and cytosolic protein, and histone H3 was used as the internal control for nuclear protein. *P<0.05 vs. the control group; ^#P<0.05 vs. the LPS group. UFH, unfractionated heparin; p38, p38 mitogen-activated protein kinase; NF-κB, nuclear factor-κB; LPS, lipopolysaccharide; IκBα, NF-κB inhibitor α; p, phosphorylated.