Figure 3.

Effect of EESB on HCT-8 cell migration and invasion. HCT-8 cells were treated with EESB at the indicated concentrations for 24 h. The (A and B) migration and (C and D) invasion of HCT-8 cells were determined using Transwell cell culture chambers and Transwell cell culture chambers with Matrigel matrix-coated membranes, respectively. (A) Migrated and (C) invaded cells were stained using crystal violet, and images were captured at a magnification of ×200. The average number of (B) migrated cells and (D) invaded cells were counted in three randomly selected fields. Data were normalized to the migration and invasion of control cells (100%). Data are expressed as the mean ± standard deviation of three independent experiments. *P<0.05 vs. the control cells. EESB, ethanol extract of Scutellaria barbata D. Don.