Figure 3.

Inhibition of iNOS and COX-2 expression by DHMDT in LPS-stimulated RAW 264.7 macrophages. The RAW 264.7 cells were pretreated with DHMDT for 1 h prior to incubation with LPS for 24 h. (A) Cell lysates were then prepared, and western blotting was performed using antibodies specific for murine iNOS and COX-2. β-actin was used as an internal control for western blot analysis. (B) ImageJ densitometric analysis of bands expressed in relation to β-actin. Data are presented as mean ± standard deviation of the mean. ^##P<0.01 vs. the untreated control; *P<0.05 and **P<0.01 vs. cells cultured with 500 ng/ml LPS only. iNOS, inducible nitric oxide synthase; COX, cyclooxygenase; DHMDT, Daehwangmokdantang; LPS, lipopolysaccharide.