Figure 5.

Effect of DHMDT on LPS-stimulated TNF-α and IL-1β expression in RAW 264.7 macrophages. The RAW 264.7 cells were pretreated with DHMDT for 1 h prior to incubation with LPS for 24 h. (A) Cell lysates were then prepared, and western blotting was performed using antibodies specific for murine TNF-α and IL-1β. β-actin was used as an internal control for western blot analysis. (B) ImageJ densitometric analysis of bands expressed in relation to β-actin. Data are presented as mean ± standard deviation of the mean. ^$P<0.05, ^##P<0.01 and ^###P<0.005 vs. the untreated control; *P<0.05 and **P<0.01 vs. cells cultured with 500 ng/ml LPS only. DHMDT, Daehwangmokdantang; LPS, lipopolysaccharide; TNF, tumor necrosis factor; IL, interleukin.