Figure 7.

Effect of DHMDT on the LPS-induced phosphorylation of IKKα/β, Akt and MAPKs in RAW 264.7 macrophages. The RAW 264.7 cells were pretreated with 800 µg/ml DHMDT for 1 h prior to exposure to LPS for 30 min, and total proteins were isolated. (A) The proteins were subjected to SDS-PAGE, followed by western blot analysis. (B) ImageJ densitometric analysis of bands expressed in relation to β-actin. Data are presented as mean ± standard deviation of the mean. *P<0.05, **P<0.01 and ***P<0.005 vs. cells cultured with 500 ng/ml LPS only. DHMDT, Daehwangmokdantang; LPS, lipopolysaccharide; p-, phosphorylated; IKK, inhibitor of nuclear factor-κB kinase; Akt, protein kinase B; ERK, extracellular signal-regulated kinase; JNK, c-Jun NH2-terminal kinase.