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Characteristics of the processed osteoblasts and bio-derived bone scaffolds. (A) Alkaline phosphatase staining of osteoblasts after purification and culturing for 3 days (magnification, ×200); (B) mineralized nodule staining of osteoblasts (magnification, ×200); (C) scanning electron microstructure of the prepared bio-derived bones (magnification, ×30; scale bar, 500 µm); (D) X-ray diffraction pattern of bio-derived bones.
