Figure 4.

Involvement of the integrin-β1 pathway in the cell/scaffold constructs. (A) MTT results of osteoblasts adhering to bio-derived bone scaffolds. Groups: 0, not treated with GRGDS; 1, treated with 1 mmol/l GRGDS. (B) Western blot results of integrin β1 in osteoblasts seeded into bio-derived bone scaffolds. Lanes: 1, Control; 2, 10 µg/ml FN-modified group; 3, 100 ng/ml bFGF-stimulated group; 4, 100 ng/ml bFGF-stimulated and 10 µg/ml FN-modified group. ***P<0.001, **P<0.01, *P<0.05, 0 vs. 1; ^###P<0.001, ^##P<0.01 among the respective GRGDS-treated groups. bFGF, basic fibroblast growth factor; FN, fibronectin; OD, optical density.