Fig. 3.
Effect of substitutions in the SpoIVFB interdomain linker during B. subtilis sporulation. B. subtilis with a wild-type spoIVFAB operon or a deletion of it at the native site, and either nothing, a spoIVFAB operon with the indicated substitution in SpoIVFB, or a wild-type spoIVFAB operon at the ectopic amyE site, were induced to sporulate, and samples collected at the indicated times postinduction were subjected to immunoblot analysis with antibodies against SpoIVFA (Top), SpoIVFB (Middle), or Pro-σK (Bottom). Sporulation was measured at 24 h after induction.