Fig. 2.

BICD2 depletion prevents HIV-1 nuclear import. Control and BICD2-depleted Jurkats, THP1 differentiated macrophages, and HeLa TZM-bl cells were subjected to synchronized infection with R7ΔEnvGFP virus pseudotyped with envelope proteins from either the JRFL (MOI 0.2), HXB2 strain (MOI 0.2), or VSV (MOI 0.4). Cells were collected 24 h postinfection and qPCR was performed using specific primers to quantify Late RT (A) and 2-LTR circles (B). Error bar represents SD of samples run in triplicate. The data shown here are representative of three or more experiments. ***P < 0.001, **P < 0.01; ns, not significant.