Fig. 5.

APOBEC1 is expressed within specific monocyte progenitors, and is required for the proper maintenance of monocyte populations in the periphery. (A) Schematic depicting the differentiation of monocytes from the cMOP. MDP, monocyte DC progenitor. (B) Apobec1 expression in sorted monocyte progenitors, relative to Gapdh expression, determined via quantitative PCR. (C) Schematic of the competitive reconstitution assay. Briefly, progenitor cells from both lineages were obtained from bone marrow and introduced into a recipient, whose progenitors were depleted via sublethal irradiation. The recipient mouse reconstitutes all blood lineages from the mixed wildtype and Apobec1^−/− progenitors. (D) Analysis of bone marrow monocyte progenitor populations in radiation chimeras reconstituted with equal numbers of wildtype and Apobec1^−/− progenitor cells 6 wk after transplantation (n = 6). (E) Analysis of spleens from mice reconstituted as in D. Error bars in D and E represent the SEM with statistical significance calculated using a t test.