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. 2017 Nov 27;114(50):13242–13247. doi: 10.1073/pnas.1710433114

Fig. 2.

Fig. 2.

L. monocytogenes-mediated delivery of the T3SS inner rod protein PrgJ activates the inflammasome in primary human macrophages. hMDMs were primed with Pam3CSK4 for 3 h and infected with PBS (mock), WT Listeria (Lm), or Lm strains expressing PrgJ and PrgI at a multiplicity of infection of five for 16 h. (A) Cell death (percentage cytotoxicity) was measured by lactate dehydrogenase release assay and normalized to mock infected cells. (B and C) IL-1α and IL-1β supernatant levels were measured by ELISA. (D) Immunoblot analysis of supernatants (sup) for mature IL-1β and lysates for pro—IL-1β and β-actin as a loading control (representative of two donors). Each data point represents the mean of triplicate infected wells for each of seven different human donors. Shaded bars represent the overall mean of the donors. *P < 0.05 by paired Wilcoxon signed rank test.