Fig. 4.
Human NAIP is required for maximal inflammasome responses to flagellin and the T3SS inner rod protein PrgJ. Primary hMDMs were transfected with control siRNA or siRNA against NAIP for 48 h and primed with Pam3CSK4 for 4 h. (A and B) Cells were treated with PA+LFn-PrgJ (PrgJTox) or PA+LFn-FlaA310–475 (FlaTox) for 5 h. (C and D) Cells were treated with LPS+Nigericin for 5 h. IL-1α and IL-1β supernatant levels were measured by ELISA. Each data point represents the mean of triplicate infected wells for six different human donors. Shaded bars represent the overall mean of the donors. NS, not significant. *P < 0.05 by paired t test; **P < 0.01 by paired t test.